Product Overview
µSFM
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Save your precious samples: 50 µl of sample is all you need.
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A unique micro-volume stopped-flow solution for the most demanding applications.
Micro-volume Stopped-flow.
Micro-volume operation is now a real requirement for stopped-flow users. Biochemists and biologists often spend days or weeks in order to prepare and purify a few hundred microliters of precious sample.
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It therefore makes sense that their time will be better spent, if this process is made as efficient as possible.
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Previously, the idea of running a complete series of experiments with only 100µl of solution was considered a pipe dream, as such volumes were necessary just for priming the instrument.
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100 µl stock, 10 shots, 1 ms dead time.
The reduction of sample consumption in stopped-flow instruments was our driving motivation for the development of the µSFM. Not a single drop of precious liquid is wasted in the stopped-flow or in the storage vial. With only a 100 µl stock solution, the user has enough solution to carry out 10 replicates. And this could even be extended to 25 replicates when carrying out refolding/folding experiments! This performance is done using our standard microcuvette so, there is no loss of sensitivity and only 1ms dead time.
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Mixing precision and modularity.
The µSFM is a two-syringe stopped-flow system. Each syringe is driven by an independent stepping motor so the mixing ratio can be freely changed in Bio-Kine software from 1:1 to 1:9. The combination of high-precision motors with the precision of micro-volume glass syringes allows the user to inject a few µl of precious samples at each experiment. A series of experiments are possible with only 50µl of stock solution and without compromising on sensitivity. Minimum injection volume can be reduced down to 3µl in asymmetric mixing conditions.
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The µSFM is a dedicated single mixing stopped-flow, optimized for absorbance, fluorescence, 90° light scattering, and X-ray kinetics. Users seeking increased modularity should consider our premium stopped-flow range.
