Freeze Quench

BioLogic's freeze quench accessory converts a Premium SFM stopped-flow instrument into a freeze quench system in a matter of minutes. Instead of measuring optically, the reacting mixture is ejected into a cold isopentane bath, freezing it so that intermediates are trapped for later offline analysis. The umbilical has a priming volume of 200 µl, flying time from nozzle to bath is under 1 ms, and freezing in isopentane takes 4 to 5 ms. Minimum ageing time in the loop is 3 ms, and the overall minimum ageing time is 9 ms. This approach is valuable for enzyme mechanism studies, where some intermediates are difficult to observe directly in solution. Because it adds to an existing SFM, labs can expand capability without buying a separate instrument. Official specifications are fairly short, so we're glad to help with additional technical detail. To discuss adding freeze quench to your stopped-flow, get in touch with ProDigitek.